We have introduced an enhanced version of this data analysis resource.

The new page features:

  • Optional Blank Correction.
  • Multiple Plate Support (process data measured across multiple plates). More...
  • Interactive Chart (to inspect data on the curve and easily mark outliers to exclude). More...
  • Enhanced Excel Output (including publication quality charting).

Click here to access the enhanced page...

(We plan to keep this existing page available for your continuing use and convenience.)

Linear Quantification

  • Email link to this assay page
  • Downloads for your assay
Download files to analyse your data independently of this page:
DownloadDownload "Linear Quantification" MyAssaysConfig file Help
Help The MyAssaysConfig file contains all the information necessary to perform analyses for this assay with the MyAssays Desktop Client. Using this tool, you can perform data analyses for your assay outside of your browser.
ELISA curve fit linear regression

Calculates the concentration of samples from a calibration curve of the standards plotted against their absorbance values. The concentration for each well is calculated from the absorbance value, constants from the linear regression and the specified dilution factors. y-int and slope are obtained from the log-regression fit of the calibration data.

Measurements outside the range of the standards are highlighted in yellow.



Supply your measurement data: Help
Help You can supply your raw data in two ways:
  1. Enter (or Paste) the raw data into the edit box. Use the same format as the example data (i.e. numbers separated by spaces). More...
  2. Select a data file on your computer and press Upload. Most raw data file formats are automatically recognised and processed. Formats not currently supported can be added very quickly. More...
After reviewing other analysis parameters press the Calculate button below to process your results.



Standard Concentrations




Sample IDs


Run Notes