Quantitative analysis of samples using linear regression.
Standard data points are plotted (concentration vs. measurement) with linear regression applied to these points. The concentrations of the samples are determined from the line with any dilution factors applied.
If the optional blank group is defined on the microplate layout, background correction is performed (the average of the blank replicates is subtracted from the raw measurement values).
Samples outside the range of the standards are highlighted in yellow.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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