We have introduced an enhanced version of this data analysis resource.

The new page features:

  • Optional Blank Correction.
  • Multiple Plate Support (process data measured across multiple plates). More...
  • Interactive Chart (to inspect data on the curve and easily mark outliers to exclude). More...
  • Enhanced Excel Output (including publication quality charting).

Click here to access the enhanced page...

(We plan to keep this existing page available for your continuing use and convenience.)

Five Parameter Logistic Fit

  • Email link to this assay page
  • Downloads for your assay
Play Video Guide
(3 min 24 sec)
Download files to analyse your data independently of this page:
DownloadDownload "Five Parameter Fit" MyAssaysConfig file Help
Help The MyAssaysConfig file contains all the information necessary to perform analyses for this assay with the MyAssays Desktop Client. Using this tool, you can perform data analyses for your assay outside of your browser.
general 5PL curve fit

Quantitative analysis of samples using a Five Parameter Logistic Fit (5PL) suitable for asymmetric sigmoidal data.

All samples are first corrected by the mean of the blank group measurements. The standard data points are plotted (concentration vs. corrected measurement) and a Five Parameter Logistic Fit (5PL) is made through these points. The concentrations of the unknown samples are determined from the fit. It is important to note that concentrations can only be determined for samples which fall within the range of the determined upper and lower asymptotes of the fit (the a and d parameters).

See also Related assays


Supply your measurement data: Help
Help You can supply your raw data in two ways:
  1. Enter (or Paste) the raw data into the edit box. Use the same format as the example data (i.e. numbers separated by spaces). More...
  2. Select a data file on your computer and press Upload. Most raw data file formats are automatically recognised and processed. Formats not currently supported can be added very quickly. More...
After reviewing other analysis parameters press the Calculate button below to process your results.



Standard Concentrations


Sample IDs


Run Notes