Quantitative measurement of AMH/MIS in human serum and other biological fluids (intended for in vitro diagnostic use only). Data analysis is performed as follows:
The mean of the blank replicates is first subtracted from the raw data measurements (the corrected values are then used in the fit).
The standard data points (concentration vs. measurement) are plotted on log axes with a cubic regression (3rd order polynomial) curve-fit. The concentrations of the samples are determined from the fit with any specified dilution factors applied.
The %CV, Standard Deviation and Standard Error are calculated for each replicated sample.
Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.
Note, when working with cubic regression, certain data sets may not be usable for valid concentration calculation. For an explanation and solution please see cubic regression.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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