Single Analyte ELISArray

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ELISA linear regression QIAGEN

Quantitative measurement of the amount of an individual cytokine or chemokine using ELISA in multiple samples.

This method subtracts the mean of the blank replicates (background absorbance) from all of the samples. The corrected absorbance is plotted against the standards concentrations on a log-log graph for a seven-point standard curve. Linear regression is used to find the best straight-line through the standards and used to determine the concentration of the unknowns. An optional dilution factor is applied.



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After reviewing other analysis parameters press the Calculate button below to process your results.



Standard Concentrations


Dilution Factors


Sample IDs


Run Notes