Quantitative measurement of the amount of an individual cytokine or chemokine using ELISA in multiple samples.
This method subtracts the mean of the blank replicates (background absorbance) from all of the samples. The corrected absorbance is plotted against the standards concentrations on a log-log graph for a seven-point standard curve. Linear regression is used to find the best straight-line through the standards and used to determine the concentration of the unknowns. An optional dilution factor is applied.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
Only members can access this function.
Please log in or join now (it's free to join).