Quantitative measurement of the amount of an individual cytokine or chemokine using ELISA in multiple samples. This method subtracts the mean of the blank replicates (background absorbance) from all of the samples. The corrected absorbance is plotted against the standards concentrations on a log-log graph for a seven-point standard curve. Linear regression is used to find the best straight-line through the standards and used to determine the concentration of the unknowns. An optional dilution factor is applied.