For the quantitative determination of human Pentraxin 3 (PTX3) concentrations in cell culture supernates, plasma, and saliva.
Analysis is performed on raw measurements at 450nm and 540nm (or 570nm). The 450nm measurements are wavelength corrected by the second measurements and the average of the blank wells is subtracted from all samples. A standard curve is plotted on a logarithmic axes and linear regression is used to calculate the concentrations of the unknowns (ng/mL). If the samples have been diluted supply the dilution factor for the unknowns.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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