For the quantitative determination of human Pentraxin 3 (PTX3) concentrations in cell culture supernates, plasma, and saliva. Analysis is performed on raw measurements at 450nm and 540nm (or 570nm). The 450nm measurements are wavelength corrected by the second measurements and the average of the blank wells is subtracted from all samples. A standard curve is plotted on a logarithmic axes and linear regression is used to calculate the concentrations of the unknowns (ng/mL). If the samples have been diluted supply the dilution factor for the unknowns.

For the quantitative determination of Colorimetric Sandwich ELISAs. This analysis calculates concentrations using a Four Parameter Logistic fit in accordance with the Quantikine® kit instructions. The average of the zero measurements is subtracted from the average of each sample. The standard data points (concentration vs. measurement) are plotted on semi-log axes and a 4PL curve is fitted through the average of the replicated standards. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

Quantitative determination of concentrations in cell culture supernates, plasma, saliva, urine, and cell lysates suitable for Parameter™ kits. All samples are corrected by the mean of the NSB group measurements. The standard data points are plotted (concentration vs. B/B0%) and a Four Parameter Logistic Fit is made through these points. The concentrations of the unknown samples are determined from the fit and any specified dilution factors are applied.

For the quantitative determination of human Vascular Endothelial Growth Factor (VEGF) concentrations in cell culture supernates, serum, and plasma. This analysis calculates concentrations using a Four Parameter Logistic fit in accordance with the kit instructions. The average of the zero measurements is subtracted from the average of each sample. The standard data points (concentration vs. measurement) are plotted on semi-log axes and a 4PL curve is fitted through the average of the replicated standards. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

For the quantitative determination of human interleukin 6 (IL-6) concentrations in cell culture supernates, serum, and plasma. This analysis calculates concentrations using a Four Parameter Logistic fit in accordance with the kit instructions. The average of the zero measurements is subtracted from the average of each sample. The standard data points (concentration vs. measurement) are plotted on semi-log axes and a 4PL curve is fitted through the average of the replicated standards. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

For the quantitative determination of of rat Interleukin 10 (IL-10) concentrations in cell culture supernates, serum, and plasma. This analysis calculates concentrations using a Four Parameter Logistic fit in accordance with the kit instructions. The average of the zero measurements is subtracted from the average of each sample. The standard data points (concentration vs. measurement) are plotted on semi-log axes and a 4PL curve is fitted through the average of the replicated standards. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

For the quantitative determination of human tumor necrosis factor alpha (TNF-a) concentrations in cell culture supernates, serum, and plasma. This analysis calculates concentrations using a Four Parameter Logistic fit in accordance with the kit instructions. The average of the zero measurements is subtracted from the average of each sample. The standard data points (concentration vs. measurement) are plotted on semi-log axes and a 4PL curve is fitted through the average of the replicated standards. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

Quantitative determination of Prostaglandin E₂ (PGE₂) concentrations in cell culture supernates, serum, plasma and urine. All samples are corrected by the mean of the NSB group measurements. The standard data points are plotted (concentration vs. B/B0%) and a Four Parameter Logistic Fit is made through these points. The concentrations of the unknown samples are determined from the fit and any specified dilution factors are applied.

Quantitative determination of Thromboxane B₂ (TXB₂) concentrations in cell culture supernates, plasma, saliva, urine, and cell lysates suitable for R&D Systems' Parameter™ kit. All samples are corrected by the mean of the NSB group measurements. The standard data points are plotted (concentration vs. B/B0%) and a Four Parameter Logistic Fit is made through these points. The concentrations of the unknown samples are determined from the fit and any specified dilution factors are applied.

For the quantitative determination of human CD163 concentrations in cell culture supernates, serum, and plasma. This analysis calculates concentrations using a Four Parameter Logistic fit in accordance with the kit instructions. The average of the zero measurements is subtracted from the average of each sample. The standard data points (concentration vs. measurement) are plotted on semi-log axes and a 4PL curve is fitted through the average of the replicated standards. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.