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R&D Systems

For the quantitative determination of Colorimetric Sandwich ELISAs.

This analysis calculates concentrations using a Four Parameter Logistic fit in accordance with the Quantikine® kit instructions.

The average of the zero measurements is subtracted from the average of each sample. The standard data points (concentration vs. measurement) are plotted on semi-log axes and a 4PL curve is fitted through the average of the replicated standards. The concentrations of the samples are determined from the fit with any specified dilution factors applied.

The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.



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Standard Concentrations


Dilution Factors


Sample IDs


Run Notes

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