Human IL-6 ELISA

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(3 min 24 sec)
eBioscience

Quantitative analysis of samples using a Five Parameter Logistic (5PL) curve fit in accordance with eBioscience Human Interleukin-6 (IL 6) ELISA kit.

This analysis optionally includes a background correction step. If a blank group is included on your layout, the mean of the blank replicates is first subtracted from the raw data measurements (the corrected values are then used in the fit).

The standard data points (concentration vs. measurement) are plotted on semi-log axes and a 5PL is fit through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.

The %CV, Standard Deviation and Standard Error are calculated for each replicated sample.

Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

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Measurements

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After reviewing other analysis parameters press the Calculate button below to process your results.
 
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Microplate

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Standard Concentrations

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Dilution Factors

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Sample IDs

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Run Notes