Competitive enzyme immunoassay for the quantitative determination of Vasopressin (AVP) [Arg8] (Human, Rat, Mouse, Ovine).
This method plots a standard curve on a semi-log graph. This curve is constructed by plotting the known concentrations of standard peptide on the log scale (X-axis), and its corresponding corrected OD reading on the linear scale (Y-axis). The standard curve shows an inverse relationship between peptide concentrations and the corresponding absorbance. As the standard concentration increases, the yellow colour decreases, thereby reducing the OD absorbance. If samples have been diluted prior to the assay, the concentration values are be multiplied by the respective dilution factors.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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