Quantitative determination of TSHR autoantibodies in human serum using a Cubic Spline for calculation of TRAb concentrations.
Raw absorbance measurements at 450nm are first corrected by the mean of the blank group measurements. The standard data points are plotted (concentration vs. corrected) and a Cubic Spline is applied to these points. The concentrations of the unknown samples are determined from the fit. Samples outside the range of the standards are marked as < 0.4 or > 30 accordingly.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
Only members can access this function.
Please log in or join now (it's free to join).