Quantification of malondialdehyde (MDA) from colorimetric measurements at 530-540nm.
This method subtracts the absorbance value of Standard1 (0 µM) from itself and all other values. The corrected absorbance values of each standard are plotted against the MDA concentrations. The MDA concentrations of the Unknown samples are determined from the linear regression.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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