In vitro enzyme-linked immunosorbent assay for the quantitative measurement of TNF-a (tumor necrosis factor-a) in serum, plasma, cell culture supernatants and urine.
This method calculates the mean absorbance for each set of replicated standards, controls and samples. The average of the zero standard is subtracted from all samples and a standard curve is plotted on log-log graph axes.
A 4PL is fitted through the plotted points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.
The %CV, Standard Deviation and Standard Error are calculated for each replicated sample.
Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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