Competitive Enzyme Immunoassay for the quantification of Visfatin peptide in accordance with RayBio® Human/Mouse/Rat Visfatin EIA Kit Protocol (Cat#: EIA-VIS-1). This method calculates B/B0% of each sample using the zero standard and the blank corrected values. The standard data points (concentration vs. B/B0%) are plotted on semi-log axes and a 4PL is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

In vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IFN-gamma in serum, plasma, cell culture supernatants and urine. This method calculates the mean absorbance for each set of replicated standards, controls and samples. The average of the zero standard is subtracted from all samples and a standard curve is plotted on log-log graph axes. A 4PL is fitted through the plotted points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

In vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-6 in serum, plasma, cell culture supernatants and urine. This method calculates the mean absorbance for each set of replicated standards, controls and samples. The average of the zero standard is subtracted from all samples and a standard curve is plotted on log-log graph axes. A 4PL is fitted through the plotted points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

In vitro enzyme-linked immunosorbent assay for the quantitative measurement of TNF-a (tumor necrosis factor-a) in serum, plasma, cell culture supernatants and urine. This method calculates the mean absorbance for each set of replicated standards, controls and samples. The average of the zero standard is subtracted from all samples and a standard curve is plotted on log-log graph axes. A 4PL is fitted through the plotted points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

Competitive Enzyme Immunoassay for the quantification of Gastric inhibitory polypeptide (GIP) in accordance with RayBio® Human/Mouse/Rat GIP EIA Kit Protocol (Cat#: EIA-GIP-1). This method calculates B/B0% of each sample using the zero standard and the blank corrected values. The standard data points (concentration vs. B/B0%) are plotted on semi-log axes and a 4PL is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.