Linearized Quantification

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EIA curve fit linear regression general

Linearized quantification of sample concentrations for enzyme immunoassay (EIA).

This uses the linearized method which plots logit B/B0 versus log concentration using a linear fit. Sample positions with %B/B0 values greater than 80% or less than 20% are highlighted in yellow. These samples should be re-assayed as they generally fall out of the linear range of the standard curve.

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Measurements

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Microplate

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Standard Concentrations

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Dilution Factors

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Sample IDs

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Run Notes