Sandwich ELISA for the quantification of Interleukin 10 (IL10).
This method plots the absorbance versus cytokine concentration on a log-log chart and performs a 5 parameter logistic curve fit through the standards.
The standard curve is used to calculate cytokine concentration of the unknown samples. If samples were diluted the concentration is multiplied by the specified dilution factor.
The %CV, Standard Deviation and Standard Error are calculated for each replicated sample.
Samples outside the range of the standards or the fit (greater than the upper asymptote or below than the lower asymptote) are highlighted in yellow.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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