Sandwich ELISA for the quantification of Interleukin 10 (IL10). This method plots the absorbance versus cytokine concentration on a log-log chart and performs a 5 parameter logistic curve fit through the standards. The standard curve is used to calculate cytokine concentration of the unknown samples. If samples were diluted the concentration is multiplied by the specified dilution factor. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below than the lower asymptote) are highlighted in yellow.