In vitro quantitative determination of bacterial endotoxin in various biological fluids (including sera), devices, (air) filters and tissue culture medium.
This data analysis calculates the mean absorbance for each set of standards, control and samples.
Validations are performed to check that the mean absorbance of the zero standard is less than 0.1 OD 405 nm and that the mean absorbance of the 10 EU/ml standard is higher than 0.6 OD 405 nm.
The mean absorbance of each standard concentration is plotted (Y axis) against the corresponding concentration (X axis) on a semi-log graph. A standard curve is created using a Four Parameter Logistic Fit. Concentrations are calculated from the curve with any specified dilution factors applied.
Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.
The %CV, Standard Deviation and Standard Error are calculated for each replicated sample.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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