Hycult Biotech LAL Chromogenic Endpoint

  • Email link to this assay page
Play Video Guide
(3 min 24 sec)
Hycult Biotech

In vitro quantitative determination of bacterial endotoxin in various biological fluids (including sera), devices, (air) filters and tissue culture medium.

This data analysis calculates the mean absorbance for each set of standards, control and samples.

Validations are performed to check that the mean absorbance of the zero standard is less than 0.1 OD 405 nm and that the mean absorbance of the 10 EU/ml standard is higher than 0.6 OD 405 nm.

The mean absorbance of each standard concentration is plotted (Y axis) against the corresponding concentration (X axis) on a semi-log graph. A standard curve is created using a Four Parameter Logistic Fit. Concentrations are calculated from the curve with any specified dilution factors applied.

Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

The %CV, Standard Deviation and Standard Error are calculated for each replicated sample.

1

Measurements

Supply your measurement data: Help
Help You can supply your raw data in two ways:
  1. Enter (or Paste) the raw data into the edit box. Use the same format as the example data (i.e. numbers separated by spaces). More...
  2. Select a data file on your computer and press Upload. Most raw data file formats are automatically recognised and processed. Formats not currently supported can be added very quickly. More...
After reviewing other analysis parameters press the Calculate button below to process your results.
 
Help
Help Select option to provide and process measurement data read across multiple plates. More...
2

Microplate

3

Standard Concentrations

4

Dilution Factors

5

Sample IDs

6

Run Notes

The Hycult Biotech name and logo are trademarks of Hycult Biotech