Direct Saliva Melatonin

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Bühlmann EIA 4PL

The Bühlmann Direct Saliva Melatonin ELISA (EK-DSM) is intended for highly sensitive, quantitative determination of melatonin in human saliva.

This method calculates the binding (B) of each pair of sample wells as a percentage of the Zero samples, with the Blank-corrected absorbance of the Incubation Buffer taken as 100%. The percentage bound (vertical axis) versus the concentration of melatonin in pg/ml (horizontal axis) is plotted on lin/log axes.

The best fit four parameter logistic curve through the standards is found and used to calculate melatonin concentration in salivary samples (pg/ml). Optional dilution values are applied to final concentration values.

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