The Bühlmann Direct Saliva Melatonin ELISA (EK-DSM) is intended for highly sensitive, quantitative determination of melatonin in human saliva.
This method calculates the binding (B) of each pair of sample wells as a percentage of the Standard1 samples, with the Blank-corrected absorbance of the Incubation Buffer taken as 100%. The percentage bound (vertical axis) versus the concentration of melatonin in pg/ml (horizontal axis) is plotted on lin/log axes.
The best fit four parameter logistic curve through the standards is found and used to calculate melatonin concentration in salivary samples (pg/ml). Optional dilution values are applied to final concentration values.
Note, in order for the Standard1 to be included in the curve fit (on a log x axis) it should be set to a low but non-zero concentration value.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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