560131 COX Inhibitor Screening

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Cayman Chemical EIA 4PL curve fit

Enzyme immunoassay (EIA) for the quantification of PGF2a.

This method plots %B/B0 for the standards versus PGF2a concentration using linear (y) and log (x) axes and performs a 4-parameter logistic fit. Sample positions with %B/B0 values greater than 80% or less than 20% are highlighted in yellow. These samples should be re-assayed as they generally fall out of the linear range of the standard curve.

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Measurements

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Microplate

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Standard Concentrations

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Dilution Factors

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Sample IDs

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Run Notes

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