Calculation of Biotinidase Enzyme Activity from absorbance measurements made at 550nm.
The substrate blank (well B1 in the default layout) is corrected by the reagent blank (well A1 in the default layout). The standards and the blanks are corrected by the reagent blank. The unknowns are corrected by their own blank which has also been corrected by the corrected substrate blank. The activity of each unknown is measured from the standard curve using linear regression.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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