Enzyme linked immunosorbent assay (ELISA) for the detection of Dog IgG1 in serum or plasma.
This method first subtracts the average OD of the blank wells from all other measurements. A standard curve is plotted and the Four Parameter Logistic Fit is performed. Concentration values are calculated from the curve and the specified dilution factors are applied.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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