Quantitative analysis of samples using a Four Parameter Logistic (4PL) curve fit with 1/Y² data suitable for AlphaLISA kits.
The standard data points (concentration vs. measurement) are plotted on log axes and a 4PL is fit through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.
The LDL is calculated by interpolating the average background counts (12 wells without analyte) + 3 x standard deviation value (average background counts + (3xSD)) on the standard curve.
The %CV, Standard Deviation and Standard Error are calculated for each replicated sample.
Samples outside the range of the standards or the fit (greater than the upper asymptote or below than the lower asymptote) are highlighted in yellow.
(Click here for an enhanced version of this tool.)
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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