Semi-quantitative ELISA for the detection of IgC class of autoantibodies specified to cyclic citrullinated peptide (CCP) in human serum (including Serum Separator Tubes) or plasma (EDTA, lithium heparin, or sodium citrate)
This method calculates concentration values using a Four Parameter Logistic Fit (4PL). Optional dilution factor values are applied to the final concentration. Unknown samples which are outside the range of the calibrators are highlighted in yellow.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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