Abcam Testosterone

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Abcam

Quantitative analysis of testosterone in serum and plasma using a Four Parameter Logistic (4PL) curve fit suitable for calculating concentrations in accordance with Abcam's in vitro competitive testosterone ELISA Kit.

This analysis optionally includes a background correction step. If a blank group is included on your layout, the mean of the blank replicates is first subtracted from the raw data measurements (the corrected values are then used in the fit).

The standard data points (concentration vs. %B/B0) are plotted on log-log axes and a 4PL is fit through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.

The %CV, Standard Deviation and Standard Error are calculated for each replicated sample.

Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote).

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Measurements

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Microplate

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Standard Concentrations

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Dilution Factors

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Sample IDs

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Run Notes