Quantitative analysis of samples using a Five Parameter Logistic (5PL) curve fit suitable for calculating concentrations in accordance with Abcam's Erythropoietin Human ELISA kit. This analysis optionally includes a background correction step. If a blank group is included on your layout, the mean of the blank replicates is first subtracted from the raw data measurements (the corrected values are then used in the fit). The standard data points (concentration vs. measurement) are plotted on log-log axes and a weighted 5PL is fit through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

Quantitative analysis of samples using a Four Parameter Logistic (4PL) curve fit suitable for calculating concentrations in accordance with Abcam's Ferritin Human ELISA kit. This analysis optionally includes a background correction step. If a blank group is included on your layout, the mean of the blank replicates is first subtracted from the raw data measurements (the corrected values are then used in the fit). The standard data points (concentration vs. measurement) are plotted on semi-log axes and a 4PL is fit through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

Quantitative analysis of testosterone in serum and plasma using a Four Parameter Logistic (4PL) curve fit suitable for calculating concentrations in accordance with Abcam's in vitro competitive testosterone ELISA Kit. This analysis optionally includes a background correction step. If a blank group is included on your layout, the mean of the blank replicates is first subtracted from the raw data measurements (the corrected values are then used in the fit). The standard data points (concentration vs. %B/B0) are plotted on log-log axes and a 4PL is fit through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied. The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote).

For the quantitative detection of ACE2 protein in human tissue extract sample. This protocol interprets the provided measurement results in accordance with Abcam's Human ACE2 SimpleStep ELISA Kit (ab235649).

For the quantitative detection of the COVID-19 N-Protein in serum and cell culture supernatants. This protocol interprets the provided measurement results in accordance with Abcam COVID-19 N-Protein ELISA Kit (ab274341).

For the quantitative detection of the COVID-19 Spike Protein in serum and cell culture supernatants. This protocol interprets the provided measurement results in accordance with Abcam COVID-19 Spike Protein ELISA Kit (ab274342).