Sigma Aldrich Ghrelin EIA

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In vitro quantitative assay for detecting Ghrelin peptide based on the principle of competitive enzyme immunoassay. Data analyis is performed in accordance with Sigma-Aldrich's Ghrelin EIA Kit.

% Absorbance is calculated for all samples from the Blank and B0 sample measurements. The standard data points are plotted (concentration vs. B/B0%) and a Four Parameter Logistic (4PL) curve is fitted through these points. The concentrations of the unknown samples are determined from the fit and any specified dilution factors are applied.



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Standard Concentrations


Dilution Factors


Sample IDs


Run Notes