In vitro quantitative assay for detecting Ghrelin peptide based on the principle of competitive enzyme immunoassay. Data analyis is performed in accordance with Sigma-Aldrich's Ghrelin EIA Kit.
% Absorbance is calculated for all samples from the Blank and B0 sample measurements. The standard data points are plotted (concentration vs. B/B0%) and a Four Parameter Logistic (4PL) curve is fitted through these points. The concentrations of the unknown samples are determined from the fit and any specified dilution factors are applied.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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