Quantitative analysis of samples using quadratic regression.
All samples are first corrected by the mean of the blank group measurements. The standard data points are plotted (concentration vs. corrected measurement) and quadratic regression is applied to these points. The concentrations of the unknown samples are determined from the fit.
To avoid ambiguous results extrapolation is not used with this method, i.e. concentration values will only be found within the range of the standards.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
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