Quantitative measure of vedolizumab.
Promonitor-VDZ is an enzyme-linked immunosorbent assay (ELISA) to be used in clinical laboratories for the quantitative detection of vedolizumab (VDZ) in human serum.
Promonitor-VDZ is a 96-well sandwich ELISA. The microwell strips are pre-coated with an anti-VDZ monoclonal antibody. Pre-diluted calibrators, controls and diluted samples are added to separate wells, allowing VDZ present to bind to pre-immobilized anti-VDZ antibodies. Unbound sample is washed away, and a second HRP-labelled anti-VDZ monoclonal antibody (conjugate) is added to each well. A second incubation step allows the conjugate to bind to the VDZ that has become attached to the microwells. After washing away the excess of unbound conjugate, the remaining enzyme activity is measured by adding a chromogenic substrate and measuring the intensity of the colour in a spectrophotometer. The signal obtained is proportional to the amount of drug in the patient sample.
Optical densities (OD) of the calibrators are plotted to construct the VDZ calibration curve. A four parameter logistic (4PL) model is used to fit the curve. Positive and Negative Controls are used to monitor assay performance. VDZ concentration is calculated by interpolating sample OD in the calibration curve. Warnings for each sample are optionally included; this setting can be configured here.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
Promonitor® is a trademark of Proteomika, S.L.
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