Promonitor-ANTI-UTK is an enzyme-linked immunosorbent assay (ELISA) to be used in clinical laboratories for the quantitative detection of anti-ustekinumab antibodies in human serum.
Ustekinumab (UTK) is a fully human immunoglobulin (Ig) G1 -kappa monoclonal antibody that binds with specificity to the p40 protein subunit used by both the interleukin (IL)-12 and 23.
Promonitor-ANTI-UTK is a 96-well bridging ELISA. The microwell strips are pre-coated with UTK. Prediluted calibrators, controls and diluted samples are added to separate wells, allowing anti-UTK antibodies present to bind to pre-immobilized UTK. Unbound sample is washed away, and a second HRP-labelled UTK (conjugate) is added to each well. A second incubation step allows the conjugate to bind to the antibodies that have become attached to the microwells. After washing away the excess of unbound conjugate, the remaining enzyme activity is measured by adding a chromogenic substrate and measuring the intensity of the color in a spectrophotometer. The signal obtained is proportional to the amount of antibodies in the patient sample.
Samples with results outside the range of the calibrators are marked > Calibrators or < Calibrators.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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