404410 Neogen 11ß-Prostaglandin F2a

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This data analysis tool calculates sample concentrations using a Four Parameter Logistic (4PL) curve fit in accordance with Neogen’s 11ß-Prostaglandin F2a (404410) kit.

This analysis optionally includes a substrate background correction step. If a blank group is defined on your layout, the mean of the blank replicates is first subtracted from the raw data measurements.

The maximal binding (%B/B0) is calculated for each sample using the B0 measurement.

The standard data points (concentration vs. %B/B0) are plotted on semi-log axes and a 4PL curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.

The %CV, Standard Deviation and Standard Error are calculated for each replicated sample.

Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.



Supply your measurement data: Help
Help You can supply your raw data in two ways:
  1. Enter (or Paste) the raw data into the edit box. Use the same format as the example data (i.e. numbers separated by spaces). More...
  2. Select a data file on your computer and press Upload. Most raw data file formats are automatically recognised and processed. Formats not currently supported can be added very quickly. More...
After reviewing other analysis parameters press the Calculate button below to process your results.



Standard Concentrations


Dilution Factors


Sample IDs


Run Notes

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