Data analysis in accordance with the In Vitro Cell Line Screening Project (IVCLSP) for the DTP anticancer drug discovery program.
This method uses seven absorbance measurements (Zero, Control growth and test growth in the presence of drug at the five concentration levels). The percentage growth is calculated at each of the drug concentrations levels. The percentage growth inhibition is plotted against the log10 concentration and point-to-point interpolation is used to calculate three dose response parameters:
GI50 - Growth inhibition of 50%
LC50 - Concentration of drug resulting in a 50% reduction in the measured protein at the end of the drug treatment as compared to that at the beginning
TGI - Drug concentration resulting in total growth inhibition
Values are calculated for each of these three parameters if the level of activity is reached; however, if the effect is not reached or is exceeded, the value for that parameter is marked in red and expressed as greater or less than the maximum or minimum concentration tested.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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