Quantitative determination of thyrotropic concentration in human serum. Data analysis is performed in accordance with Monobind's TSH kit as follows:
The absorbance values of the replicate serum references are plotted. A four parameter logistic curve is fitted to these replicated measurements. The average of each unknown group is calculated and the concentration is determined from the curve with any specified dilution factors applied.
The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
Only members can access this function.
Please log in or join now (it's free to join).