Quantitative analysis of samples using a Michaelis-Menten fit.
All samples are first corrected by the mean of the blank group measurements. The standard data points are plotted (concentration vs. corrected measurement) and a Michaelis-Menten fit is applied to these points. The concentrations of the unknown samples are determined from the fit.
The maximum rate (or Vmax) is determined from the resulting fit, this equals the asymptote. Concentrations are determined for samples outside the range of the standards using the fit.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
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