Four Parameter Logistic Curve (%Bound versus pg/ml)

  • Email link to this assay page
EIA 4PL curve fit

Enzyme immunoassay (EIA) for the quantification of concentration values of samples.

This method plots %B/B0 for the standards versus concentration using linear (y) and log (x) axes and performs a 4-parameter logistic fit. Sample positions with %B/B0 values greater than 80% or less than 20% are highlighted in yellow. These samples should be re-assayed as they generally fall out of the linear range of the standard curve.

1

Measurements

Supply your measurement data: Help
Help You can supply your raw data in two ways:
  1. Enter (or Paste) the raw data into the edit box. Use the same format as the example data (i.e. numbers separated by spaces). More...
  2. Select a data file on your computer and press Upload. Most raw data file formats are automatically recognised and processed. Formats not currently supported can be added very quickly. More...
After reviewing other analysis parameters press the Calculate button below to process your results.
 
2

Microplate

3

Standard Concentrations

4

Dilution Factors

5

Sample IDs

6

Run Notes