Four Parameter Logistic Curve (%Bound versus pg/ml)

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EIA 4PL curve fit

Enzyme immunoassay (EIA) for the quantification of concentration values of samples.

This method plots %B/B0 for the standards versus concentration using linear (y) and log (x) axes and performs a 4-parameter logistic fit. Sample positions with %B/B0 values greater than 80% or less than 20% are highlighted in yellow. These samples should be re-assayed as they generally fall out of the linear range of the standard curve.



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Standard Concentrations


Dilution Factors


Sample IDs


Run Notes