Quantitative analysis of samples using a Five Parameter Logistic (5PL) curve fit suitable for calculating concentrations from asymmetrical sigmoidal calibrators.
The sensitivity or Lowest Detection Limit (LDL) is calculated as 2 SD above the mean of the Zero replicates.
This analysis optionally includes a background correction step. If the Blank group is included on your layout, the mean of the Blank replicates is first subtracted from the raw data measurements (the corrected values are then used in the fit).
The standard data points (concentration vs. measurement) are plotted on semi-log axes and a 5PL is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.
The %CV, Standard Deviation and Standard Error are calculated for each replicated sample.
Samples outside the range of the standards or the fit (greater than the upper asymptote or below than the lower asymptote) are highlighted in yellow.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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