Determines titers of antibodies generated by immunization. In some samples there is a cross-reacting, non-specific antibody present in the serum before immunization (pre-immune Serum). This non-specific signal is subtracted out using a pool of non-immune serum. Corrected Antibody dilution curves are calculated for each unknown and used to find the dilution at a specified % of Pos Control.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
Assay design, example raw data and sample prepation notes provided by: Eva Marie Click Ph.D., Human Vaccine Institute, Duke University Medical Center.
Only members can access this function.
Please log in or join now (it's free to join).