Cytokines in mouse sera ELISA

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ELISA dilution curves

Determines titers of antibodies generated by immunization. In some samples there is a cross-reacting, non-specific antibody present in the serum before immunization (pre-immune Serum). This non-specific signal is subtracted out using a pool of non-immune serum. Corrected Antibody dilution curves are calculated for each unknown and used to find the dilution at a specified % of Pos Control.



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Dilution Factors




Sample IDs


Run Notes

Assay design, example raw data and sample prepation notes provided by: Eva Marie Click Ph.D., Human Vaccine Institute, Duke University Medical Center.