Cisbio HTRF Human IL23

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Detection of human IL23 using HTRF (an alternative to ELISA) Cisbio kit 62HIL23PEG.

IL23 is part of the response against infection, as it upregulates the expression of MMP9 (matrix metalloprotease 9), increases angiogenesis and reduces CD8 T cell infiltration into tumors. In oncology and immuno-oncology, IL23 has been studied for its effects on T cell infiltration and its promotion on TH17 cell development. IL23 is a heterodimeric cytokine composed of p19 and p40 subunits, the latter being shared with IL12. Cisbio's Human IL23 assay is highly specific for IL23 and does not cross react with IL12. The kit is a homogeneous, add and read assay to measure IL23 secretion in supernatant. It offers a fast, efficient alternative to ELISA.

This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows:

1. Delta Ratio is computed for each sample using the zero standard (Zero).

2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.

3. The %CV and Standard Deviation are calculated for each replicated sample.



Supply your measurement data: Help
Help You can supply your raw data in two ways:
  1. Enter (or Paste) the raw data into the edit box. Use the same format as the example data (i.e. numbers separated by spaces). More...
  2. Select a data file on your computer and press Upload. Most raw data file formats are automatically recognised and processed. Formats not currently supported can be added very quickly. More...
After reviewing other analysis parameters press the Calculate button below to process your results.





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