Cisbio HTRF Human IL11

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Detection of human IL11 using HTRF (an alternative to ELISA) Cisbio kit 62HIL11PEG.

IL11, member of the IL6 family of cytokines, regulates inflammatory responses and participates, according to the inflammatory microenvironnement, in cardiovascular diseases, cancers or lung fibrosis. IL-11 is produced by a variety of stromal cells, including fibroblasts, epithelial cells, and osteoblasts and has a variety of functions or effects  on hematopoiesis, also on inhibition of adipocytogenesis, osteoclast differentiation, stimulating tissue fibrosis, minimizing tissue injury, and regulating function of B cells. After its secretion, IL-11 binds to the its receptor composed by the IL11Ra chain associated with gp130 co-receptor and triggered the jak/STAT signaling pathway. HTRF human IL11 kit is a homogeneous, add and read assay, to measure IL11 secretion in cell supernatants. It offers a fast efficient alternative to ELISA.

This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows:

1. Delta Ratio is computed for each sample using the zero standard (Zero).

2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.

3. The %CV and Standard Deviation are calculated for each replicated sample.



Supply your measurement data: Help
Help You can supply your raw data in two ways:
  1. Enter (or Paste) the raw data into the edit box. Use the same format as the example data (i.e. numbers separated by spaces). More...
  2. Select a data file on your computer and press Upload. Most raw data file formats are automatically recognised and processed. Formats not currently supported can be added very quickly. More...
After reviewing other analysis parameters press the Calculate button below to process your results.





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