Cisbio HTRF Human IL10

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Cisbio

Detection of human IL10 using HTRF (an alternative to ELISA) Cisbio kit 62HIL10PEG.

Also known as CSIF (Cytokine synthesis inhibitory factor), IL10 is an anti-inflammatory cytokine. Its negative regulation of the immune response is due to its ability to block the signaling of other cytokines, like IFNg, and to inhibit CD28 phosphorylation in T cells. In immuno-oncology, IL10 is known to inhibit the expansion of TH1 cells while promoting the development of Treg and macrophages. Cisbio's Human IL10 kit is a homogeneous, add and read assay to measure IL10 secretion in supernatant. It offers a fast, efficient alternative to ELISA.

This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows:

1. Delta Ratio is computed for each sample using the zero standard (Zero).

2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.

3. The %CV and Standard Deviation are calculated for each replicated sample.

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Measurements

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After reviewing other analysis parameters press the Calculate button below to process your results.
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Microplate

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Concentrations

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Dilution Factors

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Sample IDs

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Run Notes