Cisbio HTRF Human IFN alpha

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Detection of human IFN alpha using HTRF (an alternative to ELISA) Cisbio kit 62HIFNAPEG.

Mainly produced by leukocytes, IFN alpha is involved in innate immunity response to viral infection. IFN alpha activates several intra-cellular pathways in target cells, which explains its pleiotropic effects on cell differentiation, growth and immunoregulation. Cisbio's Human IFN alpha kit is a homogeneous, add and read assay to measure IFN alpha secretion in supernatant. It offers a fast, efficient alternative to ELISA.

This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows:

1. Delta Ratio is computed for each sample using the zero standard (Zero).

2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.

3. The %CV and Standard Deviation are calculated for each replicated sample.



Supply your measurement data: Help
Help You can supply your raw data in two ways:
  1. Enter (or Paste) the raw data into the edit box. Use the same format as the example data (i.e. numbers separated by spaces). More...
  2. Select a data file on your computer and press Upload. Most raw data file formats are automatically recognised and processed. Formats not currently supported can be added very quickly. More...
After reviewing other analysis parameters press the Calculate button below to process your results.





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