Detection of human CXCL10 using HTRF (an alternative to ELISA) Cisbio kit 62HCX10PEG.
Also called IP-10 (interferon gamma-induced protein 10) or small-inducible cytokine B10, CXCL10 is a chemokine secreted by monocytes, fibroblasts and endothelial cells in response to IFN gamma. CXCL10's main effect is to promote cell migration through Calcium signaling. Target cells include macrophages, T cells, NK cells and dendritic cells. CXCL10 has significant anti-tumor effects as well as anti-angiogenic effects. Cisbio's Human CXCL10 kit is a homogeneous, add and read assay to measure CXCL10 secretion in supernatant. It offers a fast, efficient alternative to ELISA.
This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows:
1. Delta Ratio is computed for each sample using the zero standard (Zero).
2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.
3. The %CV and Standard Deviation are calculated for each replicated sample.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
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