Cisbio HTRF Human CCL2

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Detection of human CCL2 using HTRF (an alternative to ELISA) Cisbio kit 62HCCL2PEG.

Also called MCP-1 (monocyte chemoattractant protein 1), CCL2 is a chemokine mainly secreted by monocytes, macrophages and dendritic cells. Its main role is to attract monocytes, basophils and T cells. CCL2 is involved in pathologies involving monocytic infiltrates like psoriasis or atherosclerosis. In the brain, CCL2 is released by neurons, astrocytes and microglia and is part of the neuroinflammatory response observed in epilepsy, ischemia or Alzheimer’s disease. CCL2 is also secreted by adipocytes and can interfere with insulin signaling, thus suggesting a role in the inflammation linked to Diabetes. Cisbio's Human CCL2 kit is a homogeneous, add and read assay to measure CCL2 secretion in supernatant. It offers a fast, efficient alternative to ELISA.

This data analysis tool performs quantitative analysis of samples using a 1/y² weighted Four Parameter Logistic (4PL) curve fit. Provide your ratio calculated measurement data (665nm/620nm). Calculations are performed as follows:

1. Delta Ratio is computed for each sample using the zero standard (Zero).

2. The standard data points (Concentration vs. Delta Ratio) are plotted and the curve is fitted through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.

3. The %CV and Standard Deviation are calculated for each replicated sample.



Supply your measurement data: Help
Help You can supply your raw data in two ways:
  1. Enter (or Paste) the raw data into the edit box. Use the same format as the example data (i.e. numbers separated by spaces). More...
  2. Select a data file on your computer and press Upload. Most raw data file formats are automatically recognised and processed. Formats not currently supported can be added very quickly. More...
After reviewing other analysis parameters press the Calculate button below to process your results.





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