Enzyme linked immunosorbent assay (ELISA) for the quantitative measurement of Inhibin B in serum and lithium heparin plasma.
This method calculates the mean absorbance for each standard (calibrator), control and sample. The blank corrected mean absorbances are plotted against the concentration values on log axes. Cubic regression is used to determine the concentration of the samples. Any specified dilution factors are applied.
Samples are outside the range of the standards are highlighted in yellow and should be reassayed in accordance with the kit instructions.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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