Quantitative measure of adalimumab.
Promonitor®-ADL is an enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of adalimumab (ADL) in human serum samples. The amount of ADL, when considered in conjunction with anti-ADL antibodies (Promonitor®-anti-ADL Ref. 5090230000) and clinical findings, like disease activity, contributes valuable information to the physician to assess the efficacy of therapies with ADL and loss of clinical response by the patient. The correlation between reduced serum ADL concentrations, anti-IFX antibodies formation and increased disease activity has been demonstrated in several clinical studies in a series of pathologies.
Promonitor®-ADL is a sandwich ELISA where microwell strips are provided pre-coated with an anti-ADL human F(ab´)2 fragment. Diluted calibrators, controls and diluted patient samples are added to separate wells, allowing ADL present to bind to pre-immobilized anti-ADL antibodies. Unbound sample is washed away, and a second HRP-labeled anti-ADL monoclonal antibody is added to each well. A second incubation step allows the HRP-labeled anti-ADL antibody to bind to the ADL that has become attached to the microwells. After washing away the excess of unbound HRP-labeled anti-ADL antibody, the remaining enzyme activity is measured by adding a chromogenic substrate and measuring the intensity of the color that develops in a spectrophotometer. The signal obtained is proportional to the amount of drug in the patient sample.
Optical densities (OD) of the calibrators are plotted to construct the ADL calibration curve. A four parameter logistic (4PL) model is used to fit the curve. Positive and Negative Controls are used to monitor assay performance. ADL concentrations are calculated by interpolating sample OD in the calibration curve. Therapeutical interpretation of ADL results in a clinical setting is provided. Warnings for each sample are optionally included; this setting can be configured here.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
Promonitor® is a trademark of Proteomika, S.L.
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