Arbor Assays Hemoglobin Colorimetric Detection Kit - HS FORMAT

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Arbor Assays

This data analysis calculates concentrations using a Four Parameter Logistic (4PL) curve fit in accordance with Arbor Assays' Hemoglobin Colorimetric Detection Kit - HS FORMAT (K013-HX1, K013-HX5).

The average of the zero measurements is subtracted from each sample. The standard data points are plotted (concentration vs. OD) on semi-log axes and a 4PL curve is fitted through these points. The concentrations of the unknown samples are determined from the fit and any specified dilution factors are applied.

The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

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Measurements

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Microplate

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Standard Concentrations

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Dilution Factors

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Sample IDs

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Run Notes