Arbor Assays Estradiol Serum EIA kit

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Arbor Assays

This data analysis calculates concentrations using a Four Parameter Logistic (4PL) curve fit in accordance with Arbor Assays' Estradiol Serum EIA kit (KB30-H1, KB30-H5, KB30-H, KB30).

All samples are corrected by the mean of the NSB group measurements. The standard data points are plotted (concentration vs. %B/B0) on semi-log axes and a 4PL curve is fitted through these points. The concentrations of the unknown samples are determined from the fit and any specified dilution factors are applied.

The %CV, Standard Deviation and Standard Error are calculated for each replicated sample. Samples outside the range of the standards or the fit (greater than the upper asymptote or below the lower asymptote) are highlighted in yellow.

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Measurements

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After reviewing other analysis parameters press the Calculate button below to process your results.
 
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Microplate

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Standard Concentrations

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Dilution Factors

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Sample IDs

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Run Notes