Antibody titer calculations using linear regression on a specified range of dilution steps.
The average background absorbance (Blank1) is first subtracted from the measured absorbance of each sample.
This background corrected measurements (y-axis) are plotted against the log10-transformed dilution steps (x-axis). Linear regression is performed on the specified section of the resulting plot.
The average of the values measured for the Secondary Antibody Only are plotted as a horizontal line. The titer for each sample is calculated as the x-coordinate for the intersection between the regression line and the Secondary Antibody Only average.
If the entire plate was read then supply the measured value for each well. Alternatively, if only specific parts of the plate were read then list only those measured values. In this case an additional step is required: under the Microplate section below, select or create a layout which defines which wells were read and which were not. More...
A zero concentration value has been specified with a logarithmic x axis.
This is valid but means that you will not see the zero standard on the chart, also in certain cases it may result in a skewed fit. One alternative is to use a suitably low value, such as 0.01 instead of zero. More...
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